Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression response of Populus tremuloides cell suspension cultures to salicyl alcohol feeding


ABSTRACT: Aspen cell cultures were fed 5 mM salicyl alcohol at the early exponential phase (5 days after subculture). Cells were harvested from fed (SL) or unfed (C) cells after 48 hours. Gene expression changes were analyzed using a custom 7K aspen EST array. RNA was extracted from 3 independent control (C) and 3 salicyl alcohol-fed (SL) samples, and one control and one treated sample were randomly paired for hybridization (3 biological replicates). Dye-swap hybridizations were carried out for each pair of samples.

ORGANISM(S): Populus tremuloides

SUBMITTER: Chung-Jui Tsai 

PROVIDER: E-GEOD-18360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Glycosylation-mediated phenylpropanoid partitioning in Populus tremuloides cell cultures.

Payyavula Raja S RS   Babst Benjamin A BA   Nelsen Matthew P MP   Harding Scott A SA   Tsai Chung-Jui CJ  

BMC plant biology 20091229


<h4>Background</h4>Phenylpropanoid-derived phenolic glycosides (PGs) and condensed tannins (CTs) comprise large, multi-purpose non-structural carbon sinks in Populus. A negative correlation between PG and CT concentrations has been observed in several studies. However, the molecular mechanism underlying the relationship is not known.<h4>Results</h4>Populus cell cultures produce CTs but not PGs under normal conditions. Feeding salicyl alcohol resulted in accumulation of salicins, the simplest PG,  ...[more]

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