Transcription profiling of chicken bursal cells treated with stem cell antigen 2

ABSTRACT: The differentiation and proliferation of chicken B cells rely on the antigens on the cell surface of bursal stromal cells. Chicken stem cell antigen 2 (SCA2) is localized on the surface of bursal cortical and medullary epithelial cells. The signals through SCA2 may regulate the expressions critical for the B cell development. The altered expressions of bursal cells that were induced through SCA2-signaling were detailed via Affemetrix array experiments. Experiment Overall Design: Bursa of 3 week old line 0 chickens were collected and manually teased to harvest the bursal cells. The bursal cells were spread on the monolayers of SCA2Flag-DF1 cells or control Vector-DF1 cells in triplicate. After growth in the mixture of Liebovitz’s L-15 and McCoy 5A media (1:1) supplemented with 4% FBS, pen

ORGANISM(S): Gallus gallus

SUBMITTER: Weifeng Mao 

PROVIDER: E-GEOD-18506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress