P. aeruginosa PA14 gene expression in artificial sputum medium (ASMDM)

ABSTRACT: Pseudomonas aeruginosa airway infection is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. In vitro models that closely mimic CF sputum are needed to improve understanding of the pathobiology of P. aeruginosa in the CF airway. We developed an artificial sputum medium (ASMDM) that more closely resembles the composition of CF sputum than current media. In order to validate the utility of ASMDM, we used GeneChip microarrays to compare expression data of P. aeruginosa UCBPP-PA14 (PA14) in ASMDM with published data for this strain grown under the same conditions in an artificial medium containing 10% (v/v) CF sputum. Thirty-seven of 39 nutrition-related genes were differentially expressed in the same manner in both media. However, 24 quorum-sensing (QS) genes, 23

ORGANISM(S): Pseudomonas aeruginosa UCBPP-PA14

SUBMITTER: Daniel Hassett 

PROVIDER: E-GEOD-18594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress