Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Saccharomyces cerevisiae cells: effect of Top2 depletion without accumulation of positive superhelical stress


ABSTRACT: Positive DNA helical stress accumulates in vivo by the unbalanced relaxation of positive and negative DNA supercoils in Δtop1, top2ts, pGPD:TopA yeast cells. The resulting overwinding of DNA greatly diminishes overall RNA synthesis. Here we show that whereas most genes reduce their transcript levels by several fold, genes situated at less than 100 kb from the chromosomal ends (near 15% of the genome) are gradually unaffected. This positional effect denotes that chromosomal ends are topologically open, thus precluding the accumulation of DNA helical stress in telomere-proximal regions. The progressive escape from the transcription stall observed in all the chromosome extremities indicates also that friction restrictions to DNA twist diffusion, rather than tight topological boundaries, suffice to confine DNA helical tension along eukaryotic chromatin. Two-condition experiment, JCW25 vs top2-ts-TOPA (JCW26). 3 biological replicates per condition and time point, independently grown in leu- selective media and harvested.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Benjamin Piña 

PROVIDER: E-GEOD-18605 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Positional dependence of transcriptional inhibition by DNA torsional stress in yeast chromosomes.

Joshi Ricky S RS   Piña Benjamin B   Roca Joaquim J  

The EMBO journal 20100107 4


How DNA helical tension is constrained along the linear chromosomes of eukaryotic cells is poorly understood. In this study, we induced the accumulation of DNA (+) helical tension in Saccharomyces cerevisiae cells and examined how DNA transcription was affected along yeast chromosomes. The results revealed that, whereas the overwinding of DNA produced a general impairment of transcription initiation, genes situated at <100 kb from the chromosomal ends gradually escaped from the transcription sta  ...[more]

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