TimEX from human embryonic stem cells, mesenchymal stem cells, and erythroid cells

ABSTRACT: The organization of mammalian DNA replication is poorly understood. We have produced genome-wide high-resolution dynamic maps of the timing of replication in human erythroid, mesenchymal and embryonic stem cells using TimEX, a method that relies on gaussian convolution of massive, highly redundant determinations of DNA copy number variations during S phase obtained using either high-density oligonucleotide tiling arrays or massively-parallel sequencing to produce replication timing profiles. We show that in untransformed human cells, timing of replication is highly regulated and highly synchronous, and that many genomic segments are replicated in temporal transition regions devoid of initiation where replication forks progress unidirectionally from origins that can be hundreds of kilobases

ORGANISM(S): Homo sapiens

SUBMITTER: Eric Bouhassira 

PROVIDER: E-GEOD-18679 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress