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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Time course analysis of dedifferentiation in porcine follicular granulosa cells

ABSTRACT: Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a M-bM-^@M-^Xstem cellM-bM-^@M-^Y-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation of GC using global gene expression analyses. Total RNA was isolated from GCs to DFOG cells at 5 time points during dedifferentiation (cultured in 0day, 1day, 2day, 4day and 7day). Each timepoint was performed in triplicate (ie, biological replicates). Using Affymetrix porcine genome array, we performed microarray time course experiments to analyze gene expression profiles during GC dedifferentiation.

ORGANISM(S): Sus scrofa

SUBMITTER: Hiromasa Ono

PROVIDER: E-GEOD-18854 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dedifferentiated follicular granulosa cells derived from pig ovary can transdifferentiate into osteoblasts.

Oki Yoshinao Y Ono Hiromasa H Motohashi Takeharu T Sugiura Nobuki N Nobusue Hiroyuki H Kano Koichiro K

The Biochemical journal 20121001 2

Transdifferentiation is the conversion of cells from one differentiated cell type into another. How functionally differentiated cells already committed to a specific cell lineage can transdifferentiate into other cell types is a key question in cell biology and regenerative medicine. In the present study we show that porcine ovarian follicular GCs (granulosa cells) can transdifferentiate into osteoblasts in vitro and in vivo. Pure GCs isolated and cultured in Dulbecco's modified Eagle's medium s ...[more]

PMID: 22839299

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Comparative transcriptome analysis of dedifferentiation in porcine mature adipocytes and follicular granulosa cells

Project description:Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a M-bM-^@M-^Xstem cellM-bM-^@M-^Y-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that mature adipocytes (MA) and follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation mechanism of MA and GC using global gene expression analyses. Using Affymetrix porcine genome array, we compared global gene expression profiles during dedifferentiation to search for particular biological functions in genes of which expression intensities were increased or decreased by MA and GC dedifferentiation.

2011-03-10 | E-GEOD-17264 | biostudies-arrayexpress

Time course analysis of dedifferentiation in porcine follicular granulosa cells

Project description:Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a ‘stem cell’-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation of GC using global gene expression analyses.

2012-11-21 | GSE18854 | GEO

Comparative transcriptome analysis of dedifferentiation in porcine mature adipocytes and follicular granulosa cells

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2013-12-04 | E-GEOD-52871 | biostudies-arrayexpress

Polycomb group gene E(z) is required for spermatogonial dedifferentiation in Drosophila adult testis

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Cardiomyocyte Dedifferentiation and Proliferation Revealed by Massive Parallel Single-Nucleus Transciprotmic Analysis in Adult Mouse Hearts

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2019-07-02 | GSE128628 | GEO

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