Transcriptional Profile Analysis of RPGRORF15 frameshift mutation

ABSTRACT: Purpose: To identify genes and molecular mechanisms associated with disease progression during (7 weeks of age) and after (16 weeks) the peak of photoreceptor death in dogs affected with XLPRA2, a canine model of early-onset XLRP caused by a microdeletion in RPGR exon ORF15. Methods: Expression profiles of diseased and normal dog retinas at both ages were compared using a canine retinal custom cDNA microarray. Data normalization and filtering were performed with GeneSpring, statistical analysis with Significance Analysis of Microarrays. Real-time PCR using Taqman probes, western blot and immunohistochemistry (IHC) were applied on selected genes to confirm and expand the microarray results. Results: At 7 and 16 weeks, respectively, 56 and 18 transcripts were down-regulated in mutant retinas

ORGANISM(S): Canis lupus familiaris

SUBMITTER: Gustavo Aguirre 

PROVIDER: E-GEOD-19124 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress