An O-acetylserine(thiol)lyase homolog regulates cysteine homeostasis in Arabidopsis thaliana

ABSTRACT: Cysteine occupies a central position in plant metabolism due to its biochemical functions. Arabidopsis thaliana cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes that catalyze the biosynthesis of cysteine. Because they are localized in the cytosol, plastids and mitochondria, this results in multiple subcellular cysteine pools. Much progress has been made on the most abundant OASTL enzymes; however, information on the less abundant OASTL-like proteins has been scarce. To unequivocally establish the enzymatic reaction catalyzed by the minor cytosolic OASTL isoform CS-LIKE (AT5G28030), we expressed this enzyme in bacteria and characterized the purified recombinant protein. Our results demonstrate that CS-LIKE catalyzes the desulfuration of L-cysteine to sulfide plus ammonia a

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Cecilia Gotor 

PROVIDER: E-GEOD-19244 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress