In silico enhanced restriction enzyme based methylation analysis of the human glioblastoma genome

ABSTRACT: Genome wide methylation profiling of gliomas is likely to provide important clues to improving treatment outcomes. Restriction enzyme based approaches have been widely utilized for methylation profiling of cancer genomes and will continue to have importance in combination with higher density microarrays. With the availability of the human genome sequence and microarray probe sequences, these approaches can be readily characterized and optimized via in silico modeling. We adapted the previously described HpaII/MspI based Methylation Sensitive Restriction Enzyme (MSRE) assay for use with two-color Agilent 244K CpG island microarrays. In this assay, fragmented genomic DNA is digested in separate reactions with isoschizomeric HpaII (methylation-sensitive) and MspI (methylation-insensitive) r

ORGANISM(S): Homo sapiens

SUBMITTER: Anh Tran 

PROVIDER: E-GEOD-19363 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress