Expression data identifying hypermethylated genes associated with acquired cisplatin resistance

ABSTRACT: Treatment-related DNA hypermethylation may play a role in creating drug resistant phenotypes by inactivating genes that are required for cytotoxicity, but there have been no genome-wide studies to systematically investigate methylation of individual genes following exposure to chemotherapy. We used microarrays and a pharmacologic unmasking protocol in isogenic cisplatin-sensitive and -resistant cell lines to identify genes that were down-regulated in cisplatin-resistant cells and could be re-activated by the DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-Aza-dC). We identified several hundred genes that were down-regulated in each resistant cell line. Of these, 30 genes were common to > 2 cell lines, and/or reported to be down-regulated in previous studies. siRNA knockdown of tw

ORGANISM(S): Homo sapiens

SUBMITTER: Constance Monitto 

PROVIDER: E-GEOD-19397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress