Analysis of microRNA transcriptome by deep sequencing of small RNA libraries of peripheral blood (seq data)

ABSTRACT: MicroRNAs are a class of small non-coding RNAs that regulate mRNA expression at the post-transcriptional level and thereby many fundamental biological processes. A number of methods, such as multiplex polymerase chain reaction, microarrays have been developed for profiling levels of known miRNAs. These methods lack ability to identify novel miRNAs and accurately determine expression at a range of concentration. Deep or massively parallel sequencing methods are providing suitable platforms for genome wide transcriptome analysis and have the ability to identify novel transcripts. The results of analysis of small RNA sequences obtained by Solexa technology of normal peripheral blood mononuclear cells, tumor cell lines K562 (chronic myelogenous leukemia) and HL60 (acute promyelogenous leukemia

ORGANISM(S): Homo sapiens

SUBMITTER: Alok Bhattacharya 

PROVIDER: E-GEOD-19812 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress