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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Polygenic and directional regulatory evolution across pathways in Saccharomyces

ABSTRACT: We assess the prevalence of polygenic evolution in pathways between the yeasts S. cerevisiae and S. bayanus. We first established short-read sequencing methods to detect cis-regulatory variation in a diploid hybrid between the species. We then formulated an analytic strategy to test for the scenario in which selective pressure in one species, to increase or decrease the activity of a pathway, has driven the accumulation of cis-regulatory variants that act in the same direction on gene expression. Application of this test revealed a variety of yeast pathways with evidence for directional regulatory evolution. Measurement of allele-specific expression of each ortholog in a diploid hybrid between Saccharomyces species

ORGANISM(S): Saccharomyces bayanus x Saccharomyces cerevisiae

SUBMITTER: Rachel Brem

PROVIDER: E-GEOD-19837 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polygenic and directional regulatory evolution across pathways in Saccharomyces.

Bullard James H JH Mostovoy Yulia Y Dudoit Sandrine S Brem Rachel B RB

Proceedings of the National Academy of Sciences of the United States of America 20100301 11

The search to understand how genomes innovate in response to selection dominates the field of evolutionary biology. Powerful molecular evolution approaches have been developed to test individual loci for signatures of selection. In many cases, however, an organism's response to changes in selective pressure may be mediated by multiple genes, whose products function together in a cellular process or pathway. Here we assess the prevalence of polygenic evolution in pathways in the yeasts Saccharomy ...[more]

PMID: 20194736

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