Gene expression data from Guthrie blood-spot cards

ABSTRACT: Background: Standard Guthrie cards have been widely used to collect blood samples from neonates for newborn screening programs, and to a lesser extent, from normal controls and patients in research studies. Ease of blood collection (small quantity and less pain), transportation, and storage are the advantages of using these cards. It is believed that RNA obtained from these samples is of low quantity and degraded quality. However, we recently discovered that approximately 3,500 expressed genes can be detected from blood spot samples using in-house made, low resolution cDNA microarrays. Here, we established a new and improved methodology to acquire gene expression profiles from blood spot cards using commercially-available high resolution microarrays. We determined the optimal number o

ORGANISM(S): Homo sapiens

SUBMITTER: Karl Dykema 

PROVIDER: E-GEOD-19919 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress