Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Multinucleated Tartrate Resistant Acid Phosphatase Positive Cells with Macrophage and Osteoclast Phenotypes in Bone

ABSTRACT: Prothrombin (PT) and osteopontin (OPN) promotes adhesion of different TRAP-positive multinucleated cells isolated from rat long bone (Hu et al. Exp Cell Res. 2008; 314: 638-50). The PT-adhering cell could represent either an immature precursor to the OPN-adherent osteoclast or constitute a distinct multinucleated cell population with a unique role in bone. Herein, phenotypic differences between PT- and OPN- cells were investigated with microarray- expression analysis. Characteristic for PT-cells was expression of innate immune response genes and scavenger receptors whereas OPN-cells expressed typical osteoclast proteins such as collagenases implicated in bone degradation. Bone cells were extracted from neonatal Sprague Dawley rat long bones. The extracted cells were attached to PT or OPN, and thereby two cell populations were isolated. mRNA was extracted from these cells and their expression was compared with the Affymetrix GeneChip Rat Gene 1.0 ST Array.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Erik Karlström

PROVIDER: E-GEOD-19950 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Isolation and phenotypic characterization of a multinucleated tartrate-resistant acid phosphatase-positive bone marrow macrophage.

Karlström Erik E Ek-Rylander Barbro B Wendel Mikael M Andersson Göran G

Experimental hematology 20101231 3

<h4>Objective</h4>Prothrombin (PT) and osteopontin (OPN) promote adhesion of bone-derived tartrate-resistant acid phosphatase (TRAP, Acp5)-positive multinucleated cells differing in size, morphology, and resorptive activity. Here we explored phenotypic and functional differences between these cells.<h4>Materials and methods</h4>Global-wide and TRAP (Acp5) promoter messenger RNA expression, ability for phagocytosis, macrophage colony-stimulating factor-dependent migration, and in situ localizatio ...[more]

PMID: 21195739

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Dataset Information

Multinucleated Tartrate Resistant Acid Phosphatase Positive Cells with Macrophage and Osteoclast Phenotypes in Bone

Publications

Isolation and phenotypic characterization of a multinucleated tartrate-resistant acid phosphatase-positive bone marrow macrophage.

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