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Pol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (I)

ABSTRACT: Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human genome. This reveals that the chromatin landscape of pol III-transcribed genes resembles that of pol II templates in many ways, although there are also clear differences. Our analysis also discovered an entirely unexpected phenomenon, namely that pol II co-localizes with the majority of genomic loci that are bound by pol III. Chip-Seq experiments for six samples: Pol III, TFIIIB, TFIIIC, H3K4me3 in HeLa cells and Pol III, S2 phos Pol II in CD4+ cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Iouri Chepelev

PROVIDER: E-GEOD-20040 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pol II and its associated epigenetic marks are present at Pol III-transcribed noncoding RNA genes.

Barski Artem A Chepelev Iouri I Liko Dritan D Cuddapah Suresh S Fleming Alastair B AB Birch Joanna J Cui Kairong K White Robert J RJ Zhao Keji K

Nature structural & molecular biology 20100425 5

Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of protein-coding gene expression, the transcription of noncoding RNA genes by RNA polymerase III (Pol III) is less well characterized. Here we profile the epigenetic features of Pol III target genes throughout the human genome. This reveals that the chromatin landscape of Pol III-transcribed genes resembles that of Pol II templates in many ways, although there are also clear differences. Our analysis al ...[more]

PMID: 20418881

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