Tracing the derivation of embryonic stem cells from the inner cell mass by single cell RNA-seq analysis

ABSTRACT: Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a single cell whole transcriptome assay. The modified amplification strategy allows us to amplify full-length cDNAs for most of the expressed genes. We show that it is feasible to get digital gene expression profiles at single cell resolution. This allows us to ask fundamental biological questions that could not be addressed previously, especially in the embyronic stem cell studues , and to understand transcriptome complexity at the resolution of a single cell. Four-cell stage embryos were recovered f

ORGANISM(S): Mus musculus

SUBMITTER: Catalin Barbacioru 

PROVIDER: E-GEOD-20187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress