Post-transcriptional regulation of stress response in a myo1? mutant of the budding yeast Saccharomyces cerevisiae

ABSTRACT: The microarrays experiments of three biological and one technical replicates were performed in YJR12 (wt) and YJR13 (myo1?) strains. YJR12 (wild type) and YJR13 (myo1?) strains were obtained as haploid segregants from a cross between YJR6 (myo1::HIS5 strain) and BY4741 (obtained from ATTC). Cultures were grown overnight at 26ºC to an optical density between 0.5-0.8 (OD600) in complete synthetic media (CSM, 2% glucose, 1X Nitrogen base) with continuous shaking at 200 rpm. RNA was extracted from ribosomal pellets using the RNeasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer’s instructions. RNA concentrations were determined by measuring absorbance at 260nm using a Nanodrop spectrophotometer (Nanodrop Technologies). The purity and integrity of the RNA was monitored using an

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: José Rodríguez-Medina 

PROVIDER: E-GEOD-20203 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress