Microarray characterization of gene expression changes in blood during acute ethanol exposure

ABSTRACT: As part of the civil aviation safety program to define the adverse effects of ethanol on flying performance, we present results of our DNA microarray analysis of samples from a timecourse study of individuals given ethanol orally, and then evaluated by breathalyzer to monitor blood alcohol content (BAC). At five blood alcohol levels, T1-T5, blood was drawn such that the samples represented 0%, 0.04%, 0.08% BAC, and return to 0.04%, and 0.02% BAC. Microarray analysis showed that changes in gene expression could be detected across the time-course. We verified these expression changes by quantitative polymerase chain reaction (qPCR). Candidate target genes identified from the microarray analysis were clustered by expression change pattern, examined for shared functions and functional network

ORGANISM(S): Homo sapiens

SUBMITTER: Doris Kupfer 

PROVIDER: E-GEOD-20489 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress