Transcription profiling of human HCT-8 cells infected with Cryptosporidium parvum sampled at multiple time points with time seriess performed in triplicate

ABSTRACT: The origin of biological samples (In vitro infection of HCT-8 cells with Cryptosporidium parvum); Monolayers of the HCT-8 cells (ATCC CCL-244, American Type Culture Collection) were cultured in RPMI 1640 medium containing 10% fetal bovine serum and additional nutritional supplements. Cells in log phase were plated at 2×106 cells per 150 mm tissue culture dish and infected with sterilised Cryptosporidium parvum oocysts (Iowa strain). Purified oocysts were suspended in phosphate buffered saline, sterilised in 33% bleach for 7 min on ice, washed in Hank's buffered saline solution, and added to HCT-8 cultures at a ratio of one oocyst per cell. Following a 2 h excystation period at 37 °C, cells were washed with warm Hank's buffered saline solution, and cells were incubated in fresh supplement

ORGANISM(S): Homo sapiens

SUBMITTER: Mingqi Deng 

PROVIDER: E-GEOD-2077 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress