Transcriptional responses of Escherichia coli to GSNO under defined chemostat conditions.

ABSTRACT: Escherichia coli strain MG1655 was grown in a New Brunswick Scientific Bioflow III Biofermentor under continuous culture (chemostat) conditions. Cells were grown in defined media containing 54 mM glycerol as the sole and limiting source of energy and carbon. The working volume was 1 litre, and the dilution rate 0.2 h-1. For aerobic growth, the air-flow rate was 1 l/min, and the dissolved oxygen tension was maintained at 40% air saturation by measuring oxygen dissolved in the culture using a Broadley James D140 OxyProbe® electrode and automated adjustment of stirring rate. Cells were grown as above to steady-state, At steady-state, GSNO was added to the chemostat culture and to the nutrient feed at a final concentration of 200 uM unless otherwise stated. Samples were taken immediately prio

ORGANISM(S): Escherichia coli

SUBMITTER: Janet Flately 

PROVIDER: E-GEOD-2095 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress