Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiles of dental follicle cells after 7 days of differentiation in vitro with BMP2, IGF2 and dexamethasone

ABSTRACT: We analysed gene expression profiles in dental follicle cells after 7 days of osteogenic differentiation with different inducers. Total RNAs were isolated from dental follicle cells after 7 days of differentiation with dexamethasone, BMP2, IGF2 and for control with standard cell culture medium

ORGANISM(S): Homo sapiens

SUBMITTER: Christian Morsczeck

PROVIDER: E-GEOD-20963 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:The current global situation points to the trend of the aging population. Along with the growth of population diseases, the pressure to develop new cell-based or drug-based therapies is increasing. One from main challenges in the field of regenerative medicine is large bones healing and osteoporosis, mainly postmenopausal women, treatment. To understand molecular processes which underlying bone development and turnover, osteogenic differentiation using specific media was applied. Differentiation process imitating real osteogenesis is associated with proteome and metabolome changes resulting in various signaling pathways activation. Proteome was profiled by ultrahigh-performance liquid chromatography and comprehensively quantified by ion mobility enhanced mass spectrometry. From 2267 reproducibly quantified proteins, 432 were differentially abundant by strict statistic and effect size criteria. Metabolome profiling was carried out nuclear magnetic resonance in resulting 27 reproducibly quantified metabolites. From them, 8 were statistically significant and differentially abundant. KEGG and MetaboAnalyst analysis indicated several metabolic pathways involved in osteogenic process. Enrichment analysis of differentialy abundant proteins reported PPAR, FoxO, IL-7 signaling pathways, thyroid hormones and steroid biosynthesis, mineral absorption, JAK-STAT and fatty acid degradation pathways as cascades with prominent impact to osteoinduction. Among them, enrichment analysis of significant metabolites showed Aminoacyl-tRNA biosynthesis as well as Valine, leucine, isoleucine metabolism together with phenylalanine, tyrosine and tryptophane metabolism as pathways which promotes osteodifferentiation. Moreover, we also selected few identified pathways which do not reported enrichment score, but have been described in the literature as majority. These cascades underline the complexity of the whole potential osteogenic mechanism. We also proposed promising targets for next validation in patient samples, what is step forward to influence of bone and/or oral defects.