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MRNA immunoprecipitated with CUGBP1 in C2C12

ABSTRACT: Dramatic changes in gene expression occur in response to extracellular stimuli and during differentiation. Although transcriptional effects are important, alterations in mRNA decay also play a major role in achieving rapid and massive changes in mRNA abundance. Moreover, just as transcription factor activity varies between different cell types, the factors influencing mRNA decay are also cell-type specific.GREs are recognized by CUGBP1, an RNA-binding protein and instability factor whose function is affected in several neuromuscular diseases. To dectect the mRNA associated with CUGBP1, we utilized RNA immunoprecipitation followed by microarray (RIP-Chip) to identify CUGBP1-associated transcripts. To identify the mRNA associated with CUGBP1, we performed the immunoprecipitation followed by microarray (RIP-Chip)

ORGANISM(S): Mus musculus

SUBMITTER: Ju Youn Lee

PROVIDER: E-GEOD-21235 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cells

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2012-01-03 | E-GEOD-27583 | biostudies-arrayexpress

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