Comprehensive analysis of orphan CpG islands identifes novel promoters with conserved DNA methylation dynamics

ABSTRACT: We report the application of single molecule-based sequencing technology in combination with CXXC affinity purifcation (CAP-seq), MBD affinity purification (MAP-seq) and chromatin immunoprecipitation (ChIP-seq) to generate reciprocal methylation and chromatin modifcation maps in human and mouse. We find that contrary to sequence based prediction methods that humans and mice possess highly equivalent compliments of CpG islands (CGIs). The majority of these CGIs are positive for the active histone modification; H3K4me3 in embryonic stem cells (ES cells) the magnitude of which is correlated with the local density of non-methylated CpG. Approximately half of the human and mouse CGIs are distal to annotated gene promoters, yet more than 40% identify unanticipated transcription start sites as de

ORGANISM(S): Mus musculus

SUBMITTER: Adrian Bird 

PROVIDER: E-GEOD-21442 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress