Transcription profiling of mouse liver from adrenalectomized (ADX) and sham-operated mice to identofy circadian expressed genes regulated by endogenous glucocorticoids

ABSTRACT: Genome-wide expression analysis of two circadian oscillatory mechanisms in the mouse liver; To identify the genes of which the circadian expression is regulated by endogenous glucocorticoids, we performed DNA microarray analysis using hepatic RNA from adrenalectomized (ADX) and sham-operated mice. Mice were housed in a 12:12 h light-dark cycle (LD12:12; lights on at zeitgeber time (ZT) 0) for at least two weeks before the day of the experiment. Liver samples were dissected, quickly frozen, and stored in liquid nitrogen. Total RNA was purified from pools of 3 animal tissues collected at each time-point using ISOGEN (Nippon Gene Co., Ltd., Japan). Hybridization to Affymetrix GeneChip (MG-U74Av2) arrays proceeded as described (Oishi K et al., J Biol Chem, 278, 41519-41527, 2003). The average

ORGANISM(S): Mus musculus

SUBMITTER: Norio Ishida 

PROVIDER: E-GEOD-2162 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress