Transcript profiling of individual twin blastomeres derived by splitting 2-cell stage murine embryos

ABSTRACT: We have examined whether twin blastomeres from 2-cell stage mouse embryos differ in mRNA content. Amplified mRNA from 12 blastomeres derived from six embryos approximately mid-way through their second cell cycle was analyzed. Probes displaying normalized values greater than 0.25 were selected and examined for consistent bias in expression within blastomere pairs. Although transcript content varied both between individual embryos and twin blastomeres, no consistent asymmetries were observed for the majority of genes. On the other hand, 769 genes displayed a greater than 1.4-fold difference in expression across 5 of 6 pairs of blastomeres and 178 genes differed across all 6 pairs. These genes separated into two groups by class discovery clustering. Of the 769 differentially expressed genes,

ORGANISM(S): Mus musculus

SUBMITTER: R Roberts 

PROVIDER: E-GEOD-21688 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress