Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microarray analysis of cross-talks between Jasmonate acid and ethylene signaling in Arabidopsis seedlings

ABSTRACT: Jasmonate and ethylene are two important plant hormones contributing plant resistance to biotic stresses synergistically. Our experimental results showed that two ethylene activated transcription factors (EIN3/EIL1) integrated both jasmonate and ethylene signaling in multiple developmental and defense events. To understand the importance of EIN3/EIL1 in jasmonate signaling, we utilized microarray analysis to identify how much the jasmonate regulated genes are governed by EIN3/EIL1. Our results indicated that EIN3/EIL1 act sequentially in a cascade of transcriptional regulation initiated by jasmonate. Compare the JA regulated genes in Col-0, coi1-2 and ein3 eil1 to identify how much transcripts are dependent on COI1 or EIN3/EIL1

ORGANISM(S): Arabidopsis

SUBMITTER: Hongwei Guo

PROVIDER: E-GEOD-21762 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Microarray analysis of cross-talks between Jasmonate acid and ethylene signaling in Arabidopsis seedlings

Project description:Jasmonate and ethylene are two important plant hormones contributing plant resistance to biotic stresses synergistically. Our experimental results showed that two ethylene activated transcription factors (EIN3/EIL1) integrated both jasmonate and ethylene signaling in multiple developmental and defense events. To understand the importance of EIN3/EIL1 in jasmonate signaling, we utilized microarray analysis to identify how much the jasmonate regulated genes are governed by EIN3/EIL1. Our results indicated that EIN3/EIL1 act sequentially in a cascade of transcriptional regulation initiated by jasmonate.

2010-05-11 | GSE21762 | GEO

Transcription profiling of Arabidopsis wild type and ein3-1eil1-1mutant seedlings grown in the dark

Project description:Ethylene is a gaseous hormone that plays important roles in plant growth, development and stress responses. EIN3 was identified as a plant-specific transcription factor and its protein level rapidly increases upon ethylene treatment. We found that activation of EIN3 and EIN3-like 1 (EIL1) is both necessary and sufficient for ethylene-induced enhancement of seedling greening, as well as repression of the accumulation of protochlorophyllide, a phototoxic intermediate of chlorophyll synthesis. Therefore, comparation of the Wt and ein3-1eil1-1 mutant etiolated seedlings' gene expression at genome wide will be helpfull for us to find the EIN3/EIL1 target genes in chlorophyll biosynthesis pathway. Here we used microarrays to detail the global gene expression under the regulation of EIN3/EIL1.Here we used microarrays to detail the global gene expression under the regulation of EIN3/EIL1. Experiment Overall Design: 4 days dark grown Wt and ein3-1eil1-1 (AT3G20770, AT2G27050) double mutant seedlings were collected for isolation RNA. Standard Affymetrix protocal and 25K Affymetrix chip (ATH1) were used for Microarry hybridization.

2009-10-27 | E-GEOD-18631 | biostudies-arrayexpress

1-aminocyclopropane-1-carboxylic acid (ACC)-induced gene expression in wild-type Col-0, mutant upl3 upl4, mutant ein3-1, and mutant upl3 upl4 ein3-1 Arabidopsis thaliana plants

Project description:The plant hormone ethylene is involved in plant developmental, stress and environmental signalling. The ethylene signalling pathway is modulated by the master transcription factor EIN3. Here we examined the involvement of proteasome-associated UPL3 and UPL4 ubiquitin ligases in ethylene-responsive gene expression. We reveal that in absence of functional UPL3 and UPL4, plants show constitutive and enhanced activation or repression of ethylene-responsive genes in an EIN3-dependent manner. Ten-day old Arabidopsis thaliana seedlings of wild-type Col-0, mutant upl3 upl4, mutant ein3-1, and mutant upl3 upl4 ein3-1 were grown on MS media in an environmental chamber with 16/8 hour day/night light regime (120 mol m-2 s-1 light intensity) and 22 degrees Celsius. Seedlings were then transferred to 6-well plates and floated on the surface of water or 50 uM 1-aminocyclopropane-1-carboxylic acid (ACC). After 3 hours seedlings were harvested and for each treatment ~50 seedlings were pooled together into a single biological repeat. In total three independent biological repeats were collected. After harvesting seedlings were briefly dried on tissue and immediately frozen in liquid nitrogen until further analysis.

2021-10-05 | E-MTAB-10964 | biostudies-arrayexpress

Microarray analysis of gene expression in the Arabidopsis rlt1-1 rlt2-1 mutant

Project description:The Arabidopsis thaliana Imitation Switch (AtISWI) subfamily of ATP-dependent chromatin remodeling factors play essential roles in plant development. Here we show that the DDT domain transcription factor RINGLET (RLT) form a complex with AtISWI. RLT1 physically interacts with CHR11 in yeast cells. Single mutants rlt1-1 and rlt2-1 show no obvious developmental defects, while the rlt1-1 rlt2-1 double mutant phenocopies the AtISWI mutant chr11-1 chr17-1. In addtion, the RLT-ISWI complex selectively represses SEPALLATA, FRUITFULL and SUPPRESSOR OF OVEREXPRESSION OF CO 1, but not AGAMOUS and PISTILLATA in leaves. Leaves from 15-day-old seedlings of wild-type Col-0 and rlt1-1 rlt2-1 were used for RNA preparation.In the experiment data,3a3b refers to rlt1-1 rlt2-1.

2010-07-29 | E-GEOD-22584 | biostudies-arrayexpress

Global gene expression profiles of Oryza sativa wild type Nipponbare and mutant coi1-13

Project description:To reveal the underlying molecular mechanism of jasmonate inhibits gibberellins signaling in rice, we performed transcriptional profiling of wild type nipponbare and mutant coi1-13 plants on a global scale using the Affymetrix GeneChip Rice Genome Array Rice young uppermost internodes were harvested and three biological repeats were performed on Nippombare (wild-type) and coi1-13 (mutant), respectively.

2011-12-01 | E-GEOD-29577 | biostudies-arrayexpress

ETHYLENE-INSENSITIVE5 encodes a 5' to 3' exoribonuclease required for posttranscriptional regulation

Project description:Ethylene is a gaseous plant growth regulator that controls a multitude of developmental and stress responses. Recently, the levels of Arabidopsis EIN3 protein, a key transcription factor mediating ethylene-regulated gene expression, have been demonstrated to increase in response to the presence of ethylene gas. Furthermore, in the absence ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F box proteins, EBF1 and EBF2 (1-3). Here, we report the identification of ETHYLENE INSENSITIVE5 as the 5’?3’ exoribonuclease XRN4. Specifically, we demonstrate that EIN5 is a component of the ethylene signal transduction cascade acting downstream of CTR1 that is required for ethylene-mediated gene expression changes. Furthermore, we find that the ethylene insensitivity of ein5 mutant plants is a consequence of the over-accumulation of EBF1 and EBF2 mRNAs resulting in the under-accumulation of EIN3 even in the presence of ethylene gas. Together, our results suggest that the role of EIN5 in ethylene perception is to antagonize the negative feedback regulation on EIN3 by promoting EBF1 and EBF2 mRNA decay, which consequently allows the accumulation of EIN3 protein to trigger the ethylene response. Keywords: total RNA profiling, proteolysis, plant hormoine, ethylene

2006-08-08 | GSE5174 | GEO

Ethylene-insensitive5 encodes a 5'->3' exoribonuclease required for regulation of EIN3-targeting F-box proteins EBF1/2

Project description:thylene is a gaseous plant growth regulator that controls a multitude of developmental and stress responses. Recently, the levels of Arabidopsis EIN3 protein, a key transcription factor mediating ethylene-regulated gene expression, have been demonstrated to increase in response to the presence of ethylene gas. Furthermore, in the absence ethylene, EIN3 is quickly degraded through a ubiquitin/proteasome pathway mediated by two F box proteins, EBF1 and EBF2 (1-3). Here, we report the identification of ETHYLENE INSENSITIVE5 as the 5'->3' exoribonuclease XRN4. Specifically, we demonstrate that EIN5 is a component of the ethylene signal transduction cascade acting downstream of CTR1 that is required for ethylene-mediated gene expression changes. Furthermore, we find that the ethylene insensitivity of ein5 mutant plants is a consequence of the over-accumulation of EBF1 and EBF2 mRNAs resulting in the under-accumulation of EIN3 even in the presence of ethylene gas. Together, our results suggest that the role of EIN5 in ethylene perception is to antagonize the negative feedback regulation on EIN3 by promoting EBF1 and EBF2 mRNA decay, which consequently allows the accumulation of EIN3 protein to trigger the ethylene response. Keywords: Arabidopsis growth regulation signal transduction

2006-07-26 | GSE5387 | GEO

Expression from dark grown Arabidopsis Wild type (Wt, Col-o) and ein3-1eil1-1 (AT3G20770, AT2G27050) mutant seedlings

2009-10-20 | GSE18631 | GEO

Expression data from Human seminal vesicles

Project description:The human seminal plasma is a potential source of biomarkers for male reproductive disorders. A tissue-profiling analysis of the main organs participating in the secretion of this body fluid was conducted to identify tissue-specific genes along the male reproductive tract. Total RNA from non pathological Human seminal vesicles were extracted and hybridized on Affymetrix microarrays. Expression signals in seminal vesicles (present dataset), prostates (GEO; GSE7307), epidydimises (GEO; GSE7808) and testicular samples (Arrayexpress; E-TABM-130) were compared to identify genes that are detected in one of these organs only.

2010-06-08 | E-GEOD-17340 | biostudies-arrayexpress

GSK-3 inhibitor treatment effect on MLL leukemia cell

Project description:Human leukemia cell line RS4.11 was treated with GSK-3 inhibitor SB216763 for 20 hours. Gene expression profiling was performed to analyze genes affected by GSK-3 inhibition. Human leukemia cell line RS4.11 was grown in vitro and treated with 10 uM GSK-3 inhibitor SB216763 or solvent DMSO for 20 hours. Total RNA was extracted and microarray analysis was performed. Two controls and three inhibitor treatment samples were analyzed.

2010-05-27 | E-GEOD-19736 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data