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Mouse MOV10L1 associates with Piwi proteins and is an essential component of the piRNA pathway

ABSTRACT: Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell-specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicase domain in mice renders both MILI and MIWI2 devoid of piRNAs. Absence of a functional piRNA pathway in Mov10l1 mutant testes causes loss of DNA methylation and subsequent de-repression of retrotransposons in germ cells. The Mov10l1 mutant males are sterile due to complete meiotic arrest. This is the first mouse mutant that expresses Piwi proteins but lacks piRNAs, suggesting that MOV10L1 is required for piRNA biogenesis and/or loading to Piwi proteins. Examination of piRNAs in Mov10l IP and differences in total small RNA profiles in RNA helicase Mov10l+/- and Mov10l-/- mutants.

ORGANISM(S): Mus musculus

SUBMITTER: Ramesh Pillai 

PROVIDER: E-GEOD-21763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mouse MOV10L1 associates with Piwi proteins and is an essential component of the Piwi-interacting RNA (piRNA) pathway.

Zheng Ke K   Xiol Jordi J   Reuter Michael M   Eckardt Sigrid S   Leu N Adrian NA   McLaughlin K John KJ   Stark Alexander A   Sachidanandam Ravi R   Pillai Ramesh S RS   Wang Peijing Jeremy PJ  

Proceedings of the National Academy of Sciences of the United States of America 20100601 26

Piwi-interacting RNAs (piRNAs) are essential for silencing of transposable elements in the germline, but their biogenesis is poorly understood. Here we demonstrate that MOV10L1, a germ cell-specific putative RNA helicase, is associated with Piwi proteins. Genetic disruption of the MOV10L1 RNA helicase domain in mice renders both MILI and MIWI2 devoid of piRNAs. Absence of a functional piRNA pathway in Mov10l1 mutant testes causes loss of DNA methylation and subsequent derepression of retrotransp  ...[more]

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