Nucleoredoxin is required for the maintenance of Wnt/β-catenin signaling in mice embryo

ABSTRACT: We previously showed that nucleoredoxin (NRX) suppresses Wnt/β-catenin signaling through its binding to Dishevelled (Dvl) (Nat Cell Biol 8, 501-508 (2006)). To clarify the in vivo role of NRX in mammals, we here generate NRX gene-knockout mice (NRX-/- mice) by homologous recombination. NRX-/- mice die around birth. Therefore, we performed microarray analyses with NRX+/+ and NRX-/- embryos of E9.5 and E11.5 stages. Surprisingly, in the genes commonly upregulated at both stages, we could not observe Wnt/β-catenin targets. Rather, several target genes for Wnt/β-catenin pathway, such as Frizzled2 and Occludin, are downregulated in NRX-/- whole embryos. Frizzled2 is a gene reportedly expressed in developmental heart. Indeed, by RT-PCR analyses we confirmed that the expression of Frizzled2, a

ORGANISM(S): Mus musculus

SUBMITTER: Hiroaki Miki 

PROVIDER: E-GEOD-21954 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress