Transcriptional profiling of ex vivo isolated mouse blood vascular and lymphatic endothelial cells

ABSTRACT: The lymphatic vascular system plays important roles in the maintenance of interstitial fluid pressure, the afferent immune response and the absorption of dietary lipids. However, the molecular mechanisms that control lymphatic vessel network maturation and function remain largely unknown. To identify novel players in lymphatic vessel function, we isolated pure populations of lymphatic and blood vascular endothelial cells from mouse intestine using fluorescence-activated high-speed cell sorting and performed transcriptional profiling. We found that the axonal guidance molecules semaphorin 3A (Sema3A) and Sema3D were specifically expressed by lymphatic vessels. Quantitative PCR of ex vivo isolated cells and immunohistochemical analysis confirmed these results. Importantly, we found that the

ORGANISM(S): Mus musculus

SUBMITTER: Giorgia Jurisic 

PROVIDER: E-GEOD-22034 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress