Identification of promoter sequence elements involved in specific recognition by the ?S subunit of bacterial RNA polymerase.

ABSTRACT: Promoter recognition by bacterial RNA polymerase is mediated by ? subunits, which assemble transiently to RNA polymerase core enzyme (E) during transcription initiation. ? subunits drive transcription of specific sets of genes by allowing RNA polymerase to interact with different promoter sequences. However, ?70, the housekeeping ? subunit, and ?S, an alternative ? subunit mainly active during slow growth and in response to cellular stresses, appear to recognize almost identical promoter sequences, raising the question of how promoter selectivity is achieved in the bacterial cell. To identify?sequence determinants for selective promoter recognition, we performed a run-off/microarray experiment (ROMA): in vitro transcription experiments were carried out with RNA polymerase saturated either

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Clelia Peano 

PROVIDER: E-GEOD-22207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress