Transcriptomic profiling of Salmonella during lag, exponential and stationary phases of growth

ABSTRACT: Transcriptional profiles during all phases of growth of Salmonella Typhimurium SL1344 were obtained and used to investigate growth phase-dependent alterations in gene expression. Viable count growth curve analysis of the growth system showed a culture lag time of 2.09 hours, and profiles at 4, 20, 40, 60, 90 and 120 minutes were measured accordingly. For comparison, profiles of the inoculum (0 min), mid-exponential (380 min), late-exponential (630 min), early-stationary (900 min) and late-stationary phase (2880 min) were also obtained. Large-scale gene-expression changes were seen, with substantial changes within 4 minutes of inoculation, indicating the speed and sophistication at which Salmonella senses its new environment during lag phase. Timecourse; 46 samples (11 timepoints, each with two biological replicates and at least two technical replicates); each sample hybridised in a two-channel hybridization against Salmonella genomic DNA as the comparator/reference, which also acted as a control for spot quality.

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344

SUBMITTER: Matthew Rolfe 

PROVIDER: E-GEOD-22294 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress