Genome-wide Measurement of RNA Secondary Structure in Yeast

ABSTRACT: The structures of RNA molecules are often important for their function and regulation, yet there are no experimental techniques for genome-scale measurement of RNA structure. Here, we describe a novel strategy termed Parallel Analysis of RNA Structure (PARS), which is based on deep sequencing fragments of RNAs that were treated with structure-specific enzymes, thus providing simultaneous in-vitro profiling of the secondary structure of thousands of RNA species at single nucleotide resolution. We apply PARS to profile the secondary structure of the mRNAs of the budding yeast S. cerevisiae and obtain structural profiles for over 3000 distinct transcripts. Analysis of these profiles reveals several RNA structural properties of yeast transcripts, including the existence of more secondary struc

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Michael Kertesz 

PROVIDER: E-GEOD-22393 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress