Effect of brain death on gene expression in liver from rhesus macaque

ABSTRACT: The majority of transplanted organs are recovered from deceased donors after brain death (BD). BD has been hypothesized to compromise organ quality in part from the activation of systemic inflammation. The objective of this study was to characterize the immune response induced by BD in a well controlled non-human primate (NHP) model. Assessment of physiologic parameters (blood pressure, heart rate, urinary output, catecholamines, and cerebral angiograms) was used to confirm BD. After 6h of BD, we monitored changes in the peripheral blood by flow cytometry, liver gene expression by microarray and liver protein expression by Western blotting and immunohistochemistry (IHC). BD was indicated by a rapid increase in blood pressure followed by hemodynamic instability, hypotension, diabetes

ORGANISM(S): Macaca mulatta

SUBMITTER: Jamie Sperger 

PROVIDER: E-GEOD-22624 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress