Proteomics

Dataset Information

71

Proteomic analysis of the spatiotemporal based molecular kinetics of acute spinal cord injury identifies a time- and segment-specific window for effective tissue repair.


ABSTRACT: Spinal cord injury (SCI) represents a major debilitating health issue with a direct socioeconomic burden on the public and private sectors worldwide. Although several studies have been conducted to identify the molecular progression of injury sequel due from the lesion site, still the exact underlying mechanisms and pathways of injury development have not been fully elucidated. In this work, based on OMICs, 3D MALDI imaging, cytokines arrays, confocal imaging we established for the first time that molecular and cellular processes occurring after spinal cord injury (SCI) are altered between the lesion proximity, i.e., rostral and caudal segments nearby the lesion (R1-C1) whereas segments distant from R1-C1, i.e., R2-C2 and R3-C3 levels co-expressed factors implicated in neurogenesis. Delay in T regulators recruitment between R1 and C1 favor discrepancies between the two segments. This is also reinforced by presence of neurites outgrowth inhibitors in C1, absent in R1. Moreover, the presence of immunoglobulins (IgGs) in neurons at the lesion site at 3 days, validated by mass spectrometry, may present additional factor that contributes to limited regeneration. Treatment in vivo with anti-CD20 one hour after SCI did not improve locomotor function and IgG expression. These results open the door of a novel view of the SCI treatment by considering the C1 as the therapeutic target.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus norvegicus  

TISSUE(S): Nerve Cord

DISEASE(S): Not Available

SUBMITTER: Maxence Wisztorski  

LAB HEAD: Michel Salzet

PROVIDER: PXD003375 | Pride | 2016-06-08

REPOSITORIES: Pride

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Publications

Proteomic Analysis of the Spatio-temporal Based Molecular Kinetics of Acute Spinal Cord Injury Identifies a Time- and Segment-specific Window for Effective Tissue Repair.

Devaux Stephanie S   Cizkova Dasa D   Quanico Jusal J   Franck Julien J   Nataf Serge S   Pays Laurent L   Hauberg-Lotte Lena L   Maass Peter P   Kobarg Jan H JH   Kobeissy Firas F   Mériaux Céline C   Wisztorski Maxence M   Slovinska Lucia L   Blasko Juraj J   Cigankova Viera V   Fournier Isabelle I   Salzet Michel M  

Molecular & cellular proteomics : MCP 20160601 8


Spinal cord injury (SCI) represents a major debilitating health issue with a direct socioeconomic burden on the public and private sectors worldwide. Although several studies have been conducted to identify the molecular progression of injury sequel due from the lesion site, still the exact underlying mechanisms and pathways of injury development have not been fully elucidated. In this work, based on OMICs, 3D matrix-assisted laser desorption ionization (MALDI) imaging, cytokines arrays, confoca  ...[more]

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