Cell of origin influences molecular and functional properties of murine induced pluripotent stem cells

ABSTRACT: Induced pluripotent stem cells (iPSCs) have been derived from various somatic cell populations through ectopic expression of defined factors. It remains unclear whether iPSCs generated from different cell types are molecularly and functionally similar. Here, we show that iPSCs obtained from fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns. Moreover, we demonstrate that cellular origin influences the in vitro differentiation potentials of iPSCs into embryoid bodies (EBs) and different hematopoietic cells. Importantly, continuous passaging of iPSCs largely attenuates these differences. Our results suggest that low-passage iPSCs retain a transient epigenetic memory of their somatic cells of origin, which manifests as differential gene expr

ORGANISM(S): Mus musculus

SUBMITTER: Maria Figueroa 

PROVIDER: E-GEOD-22827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress