Transcription profiling of human neutrophils obtained after exposure endotoxin by bronchoscopic instillation reveals differential gene expression between air space and circulating neutrophils

ABSTRACT: Although the accumulation of neutrophils in the lungs and airways is common to many inflammatory lung diseases, including acute lung injury, the alterations that neutrophils undergo as they leave the peripheral circulation and migrate into the lungs have not been well characterized. Human volunteers were exposed to endotoxin by bronchoscopic instillation. The resulting air space neutrophil accumulation and peripheral blood neutrophils were isolated 16 h later, compared with circulating neutrophils isolated before or after to the pulmonary endotoxin exposure, and compared with circulating neutrophils exposed to endotoxin in vitro. Microarray analysis was performed on air space, circulatory, and in vitro endotoxin-stimulated neutrophils. Functional analysis included the determination of neut

ORGANISM(S): Homo sapiens

SUBMITTER: Christopher Coldren 

PROVIDER: E-GEOD-2322 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress