Histone methylation and transcription factor binding during intestinal cell differentation

ABSTRACT: Cell differentiation requires epigenetic modulation of tissue-specific genes and activities of master transcriptional regulators, which are recognized for their dominant control over cellular programs. Using novel epigenomic methods, we characterized enhancer elements specifically modified in differentiating intestinal epithelial cells and found enrichment of transcription factor-binding motifs corresponding to CDX2, a master regulator of the intestine. Directed investigation revealed surprising lability in CDX2 occupancy of the genome, with redistribution from hundreds of sites occupied only in progenitors to thousands of new sites in mature cells. Knockout mice confirmed distinct Cdx2 requirements in dividing and differentiated adult intestinal cells, including responsibility for the act

ORGANISM(S): Mus musculus

SUBMITTER: Michael Verzi 

PROVIDER: E-GEOD-23436 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress