Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli

ABSTRACT: BACKGROUND:Isobutanol is a promising next generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titers. Organic solvent tolerance is a complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing and a gene expression study to elucidate genetic bases on adaptation to exogenous isobutanol stress. RESULTS:The adaptations acquired in our evolved lineages exhibit antagonistic pleiotropy between minimal and rich medium, and appear to be specific to the effects of longer chain alcohols. By examining genotypic adaptation in multiple independent lineages, we find evidence of parallel evolution in h

ORGANISM(S): Escherichia coli

SUBMITTER: YU CHEN 

PROVIDER: E-GEOD-23526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress