Expression profiling of nhp6 mutants and wildtype yeast cells (Saccharomyces cerevisiae)

ABSTRACT: The basic unit of genome packaging is the nucleosome, and nucleosomes have long been proposed to restrict DNA accessibility both to damage and to transcription. However, nucleosome number in cells was considered fixed, and no condition was described where nucleosome number was reduced. We show here that mammalian cells lacking High Mobility Group Box 1 protein (HMGB1) contain a reduced amount of core, linker and variant histones, and a correspondingly reduced number of nucleosomes. Yeast nhp6 mutants lacking NHP6A and –B proteins, which are related to HMGB1, also have a reduced amount of histones and fewer nucleosomes. Nucleosome limitation in both mammalian and yeast cells increases the sensitivity of DNA to damage, increases transcription globally, and the relative expression of about 10

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jiannis Ragoussis 

PROVIDER: E-GEOD-23711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress