Fast forward genetics: mutation mapping and identification using next-generation sequencing and enrichment of bulk segregant pools

ABSTRACT: Phenotype-driven forward genetic experiments are among the most powerful approaches for linking biology and disease to genomic elements. Although widely used in a range of model organisms, positional cloning of causal variants is still a very laborious process. Here, we describe a novel universal approach, named fast forward genetics that combines traditional bulk segregant techniques with next-generation sequencing technology and targeted genomic enrichment, to dramatically improve the process of mapping and cloning multiple mutants in a single experiment. In a two-step procedure the mutation is first roughly mapped by ‘light’ sequencing of the bulk segregant pool, followed by genomic enrichment and deep-sequencing of the mutant pool for the linked genomic region. The latter step allows f

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michal Mokry 

PROVIDER: E-GEOD-24511 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress