Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Mer1D_SK1, mer2D_SK1, mer3D_SK1, spo22D_SK1, and spo70D_SK1 on expression/splicing microarrays


ABSTRACT: mer1D_SK1, mer2D_SK1, mer3D_SK1, spo22D_SK1, spo70D_SK1 late 9h meiotic time point. mer1D, mer2D, mer3D, spo22D diploid, and spo70D diploid was generated in the SK1 strain background (ATCC: MYA-2089) by PCR-mediated cassette-based gene replacement where the MER1, MER2, MER3, SPO22, or SPO70 ORF respectively was replaced with HIS3 from S. kluyveri then the transformants were sporulated and the homozygous deletion was verified by PCR. Meiotic time point =9h; Dye swap; Reference WT_SK1 9h

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Lily Shiue 

PROVIDER: E-GEOD-24678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae.

Munding Elizabeth M EM   Igel A Haller AH   Shiue Lily L   Dorighi Kristel M KM   Treviño Lisa R LR   Ares Manuel M  

Genes & development 20101201 23


Splicing regulatory networks are essential components of eukaryotic gene expression programs, yet little is known about how they are integrated with transcriptional regulatory networks into coherent gene expression programs. Here we define the MER1 splicing regulatory network and examine its role in the gene expression program during meiosis in budding yeast. Mer1p splicing factor promotes splicing of just four pre-mRNAs. All four Mer1p-responsive genes also require Nam8p for splicing activation  ...[more]

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