Transcription profiling of mature, naive B cells to investigate how the PTPN22 autoimmune susceptibility gene affects the removal of human developing autoreactive B cells.

ABSTRACT: Protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene segregates with most autoimmune diseases; its risk allele encodes overactive PTPN22 phosphatases that alter B cell receptor (BCR) signaling potentially involved in the regulation of central B cell tolerance. To assess whether PTPN22 risk allele affects the removal of developing autoreactive B cells, we tested by ELISA the reactivity of recombinant antibodies isolated from single B cells from asymptomatic healthy individuals carrying one or two PTPN22 risk allele(s). We found that new emigrant/transitional and mature naive B cells from PTPN22 risk allele carriers contained high frequencies of autoreactive clones compared to non-carrier control donors. Hence, a single PTPN22 risk allele has a dominant effect on altering autorea

ORGANISM(S): Homo sapiens

SUBMITTER: Eric Meffre 

PROVIDER: E-GEOD-24736 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress