Effects of Rituximab on global gene expression profiles in the RA synovium

ABSTRACT: Objective: Rituximab displays therapeutic benefits in the treatment of rheumatoid arthritis (RA) patients resistant to TNF blockade. However, the precise role of B cells in the pathogenesis of RA is still unknown. In this study we investigated the global molecular effects of rituximab in synovial biopsies obtained from anti-TNF resistant RA patients before and after administration of the drug. Methods: Paired synovial biopsies were obtained from the affected knee of anti-TNF resistant RA patients before (T0) and 12 weeks after initiation of rituximab therapy (T12). Total RNA was extracted, labeled according to standard Affymetrix procedures and hybridized on GeneChip HGU133 Plus 2.0 slides. Immunohistochemistry and quantitative real-time PCR experiments were performed to confirm the diffe

ORGANISM(S): Homo sapiens

SUBMITTER: Ilse Gutierrez-Roelens 

PROVIDER: E-GEOD-24742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress