Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

10 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genomic expression study of regenerated endothelial cells in high cholesterol-fed and fish oil-rich groups

ABSTRACT: Experiments on primary cultures of native and regenerated endothelial cells demonstrated genomic changes in the latter related to vasomotor control, coagulation, oxidative stress, lipid metabolism and extracellular matrix. However, the genomic changes caused by the combination of either hyperlipidemia or supplementation with polyunsaturated fatty acids and endothelial regeneration are unknown.The present experiments were designed to test the hypothesis that endothelial regeneration process is affected differentially at the genomic level by the exposure to either high cholesterol or PUFA-rich diet in vivo. This study consists of three treatment groups, the control (n=8), high cholesterol-fed (CHL;n=6) and PUFA-rich (FO; n=6) groups. Native (LCX) and regenerated (LAD) samples of each animal from each treatment group was analyzed and followed by statistical analysis (paired t test) of all the animals from each group. The analytical results (N vs R) from each group would then be compared to that of the control group. Native cells from control group would also be compared to the native cells of the other animal groups (CHL/FO). Supplementary files: Comparisons of fish oil vs ctrl and high cholesterol vs ctrl.

ORGANISM(S): Sus scrofa

SUBMITTER: Mary Lee

PROVIDER: E-GEOD-24762 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Differential BN analysis of cytoadherence complexes in P. falciparum

Project description:The pathogenesis of severe malaria has been associated with the ability of P. falciparum infected erythrocyte binding to host endothelium (a process known as cytoadherence) but the mechanisms underlying this have not been clearly described. To understand more about the molecular components that allow the parasite to modulate the behaviour of host endothelium and interact with endothelial molecules, we have used a combined proteomic technique of protein separation (1D Blue-Native electrophoresis) and mass spectrometry to study the interaction of infected erythrocytes (IE) with endothelial cells (EC) in a co-culture system.

2018-08-09 | PXD009154 | Pride

CD44s transfection in HEK cells

Project description:In order to understand the transcriptional effects of CD44s expression in a cell line that does not express CD44 in its native form we transfected CD44s into HEK cells and measured the transcriptional chances compared to native HEK cells Three biological replicates from each condition (native HEK cells and CD44s transfected cells) were measured using Affymetrix arrays

2014-12-05 | E-GEOD-63862 | biostudies-arrayexpress

miRNA analysis of HBMVECs exposed or unexposed to U87 glioma cells

Project description:A key step in angiogenesis is the upregulation of growth factor receptors on endothelial cells. Here we demonstrate that a small regulatory microRNA, miR-296 has a major role in this process. Glioma cells and angiogenic growth factors elevate the level of miR-296 in primary human brain microvascular endothelial cells in culture. The miR-296 level is also elevated in primary tumor endothelial cells isolated from human brain tumors compared to normal brain endothelial cells. Growth factor-induced miR-296 contributes significantly to angiogenesis by directly targeting the hepatocyte growth factor-regulated tyrosine kinase substrate (HGS) mRNA, leading to decreased levels of HGS and thereby reducing HGS-mediated degradation of the growth factor receptors VEGFR2 and PDGFR-Î². Furthermore, inhibition of miR-296 with antagomirs reduces angiogenesis in tumor xenografts in vivo. Keywords: comparitive miRNA analysis 3 biological replicates (HBMVECs) are compared to 3 biological replicates (HBMVECs exposed to U87 glioma cells)

2010-06-25 | E-GEOD-13091 | biostudies-arrayexpress

MicroRNA expression data from human coronary artery endothelial cells treated with HDL components

Project description:We quantified differential microRNA (miRNA) expression in human coronary artery cells treated with native HDL, reconstituted HDL, lipid-free apolipoprotein A-I, small unilamellar vesicles, or PBS control. These data were used to determine whichmiRNAs are regulated by native HDL compared to components of HDL and categorize data based on shared sets of miRNAs and distinct sets of miRNAs regulated by each component. Each group had a n=5 and gene expression changes for each condition were compared to control PBS-treat HCAECs

2014-02-27 | E-GEOD-53315 | biostudies-arrayexpress

Expression data from human coronary artery endothelial cells treated with HDL components

Project description:We quantified differential gene (mRNA) expression in human coronary artery cells treated with native HDL, reconstituted HDL, lipid-free apolipoprotein A-I, small unilamellar vesicles, or PBS control. These data were used to determine which genes are regulated by native HDL compared to components of HDL and categorize data based on shared sets of genes and distinct sets of genes regulated by each component. Each group had a n=5 and gene expression changes for each condition were compared to control PBS-treated HCAECs

2014-02-27 | E-GEOD-53201 | biostudies-arrayexpress

The biochemical basis of mitochondrial dysfunction in Zellweger Spectrum Disorder

Project description:We identified that peroxins were still expressed in Zellweger Spectrum Disorder (ZSD) and a subset of them accumulated on the mitochondrial membrane, which resulted in gross mitochondrial abnormalities and impaired mitochondrial metabolic function. In this complexome analysis we detected several peroxins in the mitochondrial fraction in the absence of functional peroxisomes and that this accumulation is exacerbated by the loss of the mitochondrial extractase Msp1. In addition, we observed that specific peroxisomal matrix proteins localize to the mitochondria, which suggest that a functional peroxisomal docking and import complex assembles on the mitochondria in the absence of peroxisomes.

2021-06-25 | PXD024625 | Pride

Discovery of Shear- and Side-specific mRNAs and miRNAs in Human Aortic Valvular Endothelial Cells

Project description:Identify shear and side-specific miRNAs in Human Aortic Valvular Endothelial Cells using the following conditions: 1) fHAVEC exposed to OS (FO), 2) vHAVEC exposed to OS (VO), 3) fHAVEC exposed to LS (FL), and 4) vHAVEC exposed to LS (VL). 24 samples; n=6 for the following 4 groups: FO, VO, FL, and VL. 48 samples total (24 miRNA, 24mRNA)

2011-11-29 | E-GEOD-26953 | biostudies-arrayexpress

Influence of substrate stiffness on barrier function in an iPSC-derived in vitro blood-brain barrier model

Project description:Induced pluripotent stem cells were differentiated to brain microvascular endothelial-like (BMEC-like) cells and cultured on hydrogel substrates of varying stiffness. Cellular changes were measured by bulk transcriptome and proteome readouts.

2021-04-07 | E-MTAB-10336 | biostudies-arrayexpress

Bacterial cellulose carrier maintains native endothelial cell differentiation in vitro

Project description:A comprehensive transcriptomic and proteomic characterization of native HUVEC and HUVEC grown on collagen-coated Xellulin and collagen-coated conventional cell culture plastic from six donors, a total of 28 samples/libraries in 3 groups (1) Native HUVEC freshly isolated from six umbilical cords and then propagated (passage 0) (2) Ten samples of HUVEC passage 1 cultured on Xell-Discs Xellulin (3) twelve samples HUVEC passage 1 cultured in standard plastic cell culture

2016-11-30 | E-MTAB-4747 | biostudies-arrayexpress

Evolution of neuronal and endothelial transcriptomes in primates

Project description:Neurons and endothelial cells were identified by immunohistochemistry in human brains, isolated by laser-capture-microdissection and used to find genes preferentially expressed in the two cell types. Keywords: cell type comparison Laser capture microdissection was used to isolate approximately 1000 neurons and endothelial cells from 6 (for neurons) and 7 (for endothelial cells) human post mortem brain samples. RNA was isolated and amplified (3 linear amplifications) and genome wide expression was measured. This allowed the identification of genes that are differentially expressed between neurons and endothelial cells in the human brain.

2010-06-08 | E-GEOD-12293 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data