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Transcription profiling of response of Rattus norvegicus frontal cortex to chronic olanzapine treatment

ABSTRACT: Male Sprague-Dawley albino rats (Charles River) initially weighting approximately 200 grams were randomly assigned to one of the following: 1) olanzapine (2 mg/kg/day, I.P.) (N=20) or 2) Saline (N=20) and administered olanzapine or saline for 21 days. Frontal cortex (N=4/group) was dissected and homogenized for microarray, QPCR and western blotting following previous methods (Fatemi et al. 2003; Brooks et al. 2003). RNA isolation, cDNA preparation, microarray hybridization and QPCR followed previous methods (Brooks et al. 2003).

ORGANISM(S): Rattus norvegicus

SUBMITTER: S Hossein Fatemi

PROVIDER: E-GEOD-2547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chronic olanzapine treatment causes differential expression of genes in frontal cortex of rats as revealed by DNA microarray technique.

Fatemi S Hossein SH Reutiman Teri J TJ Folsom Timothy D TD Bell Christopher C Nos Lisa L Fried Peter P Pearce David A DA Singh Sushmita S Siderovski David P DP Willard Francis S FS Fukuda Mitsunori M

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 20060111 9

Recent emerging biochemical data indicate that several important neuroregulatory genes and proteins may be involved in the etiology of schizophrenia and bipolar disorder. Additionally, the same genes appear to be targets of several psychotropic medications that are used to treat these disorders. Recent DNA microarray studies show that genes involved in synaptic neurotransmission, signal transduction, and glutamate/GABA regulation may be differentially regulated in brains of subjects with schizop ...[more]

PMID: 16407901

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Olanzapine vs. Saline rat pfc

Project description:Male Sprague-Dawley albino rats (Charles River) initially weighting approximately 200 grams were randomly assigned to one of the following: 1) olanzapine (2 mg/kg/day, I.P.) (N=20) or 2) Saline (N=20) and administered olanzapine or saline for 21 days. Frontal cortex (N=4/group) was dissected and homogenized for microarray, QPCR and western blotting following previous methods (Fatemi et al. 2003; Brooks et al. 2003). RNA isolation, cDNA preparation, microarray hybridization and QPCR followed previous methods (Brooks et al. 2003). Keywords = olanzapine Keywords = frontal cortex Keywords: repeat sample

2007-04-10 | GSE2547 | GEO

Quantitative acetyl-proteome profiling of Camellia sinensis cv.‘Anji Baicha’ during periodic albinism reveal lysine acetylation of proteins involved in photosynthesis and secondary metabolite biosynthetic pathways

Project description:The tea plant (Camellia sinensis (L.) O. Kuntze) is often commercially used as a source of non-alcoholic beverages and is an economically important woody crop (Chen et al., 2007). As living standards have improved, the requirement for high-quality life has increased in modern society. ‘Anji Baicha’ (alias ‘Baiye 1’ or ‘White Leaf 1’) is an excellent tea cultivar with albino phenotype and it is popular in producing high-quality green tea. The traits of ‘Anji Baicha’ are as follows. Young ‘Anji Baicha’ shoots are yellow-green when the early spring temperature is below 20°C. As the leaves fully expand, the leaves become white. The leaves gradually return to green when the environmental temperature increases (Cheng et al., 1999; Li et al., 2002, 2011). Previous reports have suggested a positive correlation between amino acid concentration and albinism as well as a negative correlation between tea polyphenols and albinism (Li et al., 1996; Du et al., 2006; Xiong et al., 2013). Therefore, the quality of ‘Anji Baicha’ is much higher when new shoots become albino due to their rich amino acid content and modest tea polyphenol content. Plants adjust their metabolism in response to environmental stimuli to eventually bring about changes in protein activities and levels, and this adaptive process includes posttranslational protein modifications (PTMs) (Prabakaran et al., 2012). PTMs have been reported to regulate various processes, including DNA interaction, protein-protein interactions, enzyme activation and protein stability. Among the hundreds of different PTMs, lysine acetylation is an abundant, reversible and highly regulated PTM (Zhang et al., 2009; Wu et al., 2011).

2018-01-15 | PXD008134 | Pride

VCAM1 promotes outbreak from dormant bone metastasis

Project description:A weakly bone metastatic variant of the breast cancer cell line MDA-MB-231, SCP6, gave rise to highly bone metastatic sublines (PD1, PD2A-E) after long time dormancy in vivo. These cell lines were subjected to microarray analysis with data drawn from previous studies (Kang et al., 2003; Minn et al. 2005; Lu and Kang 2009; Lu and Kang 2010). Keywords: Cell type comparison

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FAIRE-chip in MCF7 and LNCaP

Project description:The compaction degree of chromatin is intimately related to its functionality and active cis-regulatory elements typically exist within open chromatin regions depleted in nucleosomes (Heintzman et al. 2007; Boyle et al. 2008). These domains can be identified using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) that allows for enrichment of nucleosome-depleted genomic regions when cross-linked chromatin is subjected to phenol-chloroform extraction (Nagy et al. 2003; Hogan et al. 2006) Here, chromatin structure was analyzed in MCF7 and LNCaP cells using Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) (Nagy et al. 2003; Hogan et al. 2006) Keywords: FAIRE-chip

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Nuclear Factor I/B is an Oncogene in Small Cell Lung Cancer

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2011-12-08 | GSE29533 | GEO

Transcriptional analysis of carbohydrate utilization by Lactobacillus acidophilus using whole-genome microarrays

Project description:L. acidophilus cells, transferred at least five times in modified MRS medium (Barrangou et al, 2003) containing the carbohydrate of interest, were grown to an OD600 of 0.6, harvested and processed for microarray experiments (Azcarate-Peril et al, 2005). Keywords: other

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Involvement of transcription factor early growth response 4 (EGR4) in lung cancer bone metastasis

Project description:EGR4 (early growth response 4), a transcription factor, was previously shown to be up-regulated in lung cancer bone metastasis, compared with its expression in metastasis in other organs, using a multiorgan metastasis model of human small-cell lung cancer cells (SBC-5) in NK cell-depleted SCID mice (Kakiuchi S et al. Mol Cancer Res 2003). Here we show that EGR4 was essential for survival cancer cells, and migration ability. Next, in searching of downstream genes regulated by EGR4, we analyzed the changes in transcriptomes of SBC-5 cells at different time-points (24, 48 and 72 hr) when knockdown of EGR4 by siRNA. Lots of genes were identified either up-regulated or down-regulated in siEGR4 samples compared with control siRNA. Among these genes, there were genes with roles in metastasis or bone biology. The fact that EGR4 knockdown led to up-regulation or suppression of these genes also indicated dual roles of EGR4 in transcriptional regulation, which is consistent with previous reports: EGR4 may repress its own promoter (Zipfel PF et al. Biochim Biophys Acta. 1997) or activate certain inflammatory genes (Decker EL et al. Nucleic Acids Res. 2003; Wieland GD et al. J Cell Sci. 2005).

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SeaIce, Espegrend, Microscosm, Mnemi

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Global analysis of lysine acetylation reveals its role in multiple biological processes in Glycine max

Project description:Glycine max was cultivated in China for nearly 5000 years, commonly referred to as soybeans, now it has become one of the important economic crops in the world (Li et al., 2008). Post-translational modifications are known to regulate many cellular processes, which are dynamic and reversible and can make protein functions changed.(Westermann and Weber, 2003). To date, among 400 PTMs have been detected, such as Acetylation, Ubiquitination, Phosphorylation, Malonylation, Succinylation and Methylation(Colak et al., 2013; Weinert et al., 2013).

2020-11-16 | PXD021246 | Pride

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Project description:Triplicates preparations of RNA from day 10 DC's. Experiment is described in Wong et al 2003 Nat. Immunol. Keywords: equivalent probe

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