Profiling of promoter occupancy by PPARM-NM-1 in human hepatoma cells via ChIP-chip analysis

ABSTRACT: The transcription factor Peroxisome Proliferator-Activated Receptor M-NM-1 (PPARM-NM-1) is an important regulator of hepatic lipid metabolism. While PPARM-NM-1 is known to activate transcription of numerous genes, no comprehensive picture of PPARM-NM-1 binding to endogenous genes has yet been reported. To fill this gap, we performed ChIP-chip in combination with transcriptional profiling on HepG2 human hepatoma cells treated with the PPARM-NM-1 agonist GW7647. We found that GW7647 increased PPARM-NM-1 binding to 4220 binding regions. GW7647-induced binding regions showed a bias around the transcription start site and most contained a predicted PPAR binding motif. Several genes known to be regulated by PPARM-NM-1, such as ACOX1, SULT2A1, ACADL, CD36, IGFBP1 and G0S2, showed GW7647-induced P

ORGANISM(S): Homo sapiens

SUBMITTER: Guido Hooiveld 

PROVIDER: E-GEOD-25547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress