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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Circadian transcriptional profiling of peritoneal macrophages

ABSTRACT: In the immune system various parameters and immune functions are controlled by the circadian system. To investigate molecular mechanisms that link the circadian clock and the immune system we analyzed the transcriptom of peritoneal macrophages from mice collected in a time course for two consecutive days. We found that more than 8% of expressed genes are under circadian control including many important regulators in pathogen recognition, signal transduction and cytokine secretion. Peritoneal macrophages from mice kept in constant conditions were isolated in 4 h intervalls over a period of 48 h. RNA of CD11b sorted cells was extracted, pooled from 3 mice per time point and used for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Bert Maier

PROVIDER: E-GEOD-25585 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A circadian clock in macrophages controls inflammatory immune responses.

Keller Maren M Mazuch Jeannine J Abraham Ute U Eom Gina D GD Herzog Erik D ED Volk Hans-Dieter HD Kramer Achim A Maier Bert B

Proceedings of the National Academy of Sciences of the United States of America 20091201 50

Time of day-dependent variations of immune system parameters are ubiquitous phenomena in immunology. The circadian clock has been attributed with coordinating these variations on multiple levels; however, their molecular basis is little understood. Here, we systematically investigated the link between the circadian clock and rhythmic immune functions. We show that spleen, lymph nodes, and peritoneal macrophages of mice contain intrinsic circadian clockworks that operate autonomously even ex vivo ...[more]

PMID: 19955445

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Circadian transcriptional profiling of peritoneal macrophages

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