Transcriptional profiling of Escherichia coli during a transition from zinc starvation to surfeit

ABSTRACT: Escherichia coli strain MG1655 was grown in a Zn-depleted custom-built chemostat. Culture volume (120 ml), temperature (37 oC) and stirring speed (440 rpm) were maintained. Steady state values for pH and OD600 were 6.9 and 0.6, respectively. Chemostats were grown for 50 h to allow five culture volumes to pass through the vessel and allow an apparent (pseudo-)steady state to be reached. At this point, ZnSO4.7H2O in water was added to a final concentration of 0.2 M in the chemostat. A 10 ml sample of culture was taken using a polypropylene pipette tip immediately prior to Zn addition and 2.5, 7, 10 and 30 min after addition. Samples were harvested into RNAprotect and total RNA was purified using QiagenM-bM-^@M-^Ys RNeasy Mini kit (using the supplierM-bM-^@M-^Ys protocol) prior to use i

ORGANISM(S): Escherichia coli

SUBMITTER: Robert Poole 

PROVIDER: E-GEOD-26187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress