Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium

ABSTRACT: Thermococcus gammatolerans, the most radioresistant archaeon known to date, is an anaerobic and hyperthermophilic sulfur-reducing organism living in deep-sea hydrothermal vents. Knowledge of mechanisms underlying archaeal metal tolerance in such metal-rich ecosystem is still poorly documented. We showed that T. gammatolerans exhibits high resistance to cadmium (Cd), cobalt (Co) and zinc (Zn), a weaker tolerance to nickel (Ni), copper (Cu) and arsenate (AsO4) and that cells exposed to 1mM Cd exhibit a cellular Cd concentration of 66M-BM-5M. A time-dependent transcriptomic analysis using microarrays was performed at a non-toxic (100M-NM-3 with a maximum of almost 10 for one encoding a conserved hypothetical protein (tg0885, 1mM Cd at 120min), the large majority of the up- and down-regulated genes exhibited a 2 to 3-fold transcriptional change as already described in many archaeal transcriptomic studies.

ORGANISM(S): Thermococcus gammatolerans

SUBMITTER: Arnaud Lagorce

PROVIDER: E-GEOD-13546 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium

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