Study the effect of mesenchymal stem cells on isolated cortical neurons before and after NMDA treatment

ABSTRACT: Primary cortical neurons were isolated from E15 mice and after 5 days in vitro were untreated or treated for 24 h with mesenchymal stem cell conditioned medium and then untreated or treated for a further 24 h with NMDA. Neuron gene expression was profiled and compared between the four different conditions (neurons, neurons+MSC cm, neurons+NMDA, neurons+MSC cm+NMDA) to investigate the molecular mechanisms of MSC neuroprotection. Mesenchymal stem cells (MSC) promote functional recovery in experimental models of central nervous system (CNS) pathology and are currently being tested in clinical trials for stroke, multiple sclerosis and CNS injury. Their beneficial effects are attributed to activation of endogenous CNS repair processes and immune regulation but their mechanisms of action are poo

ORGANISM(S): Mus musculus

SUBMITTER: Vivian Tseveleki 

PROVIDER: E-GEOD-26279 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress